This question has couple parts; please answer all parts Explain figure 8.19 in y

Question

This question has couple parts; please answer all parts

Explain figure 8.19 in your own words. What questions do you have about this reading? In which parts of this figure 8.19, find yourself feeling most interested & you find yourself feeling confused? What questions did it bring up? What did it make you wonder?

Gene conferring StrS Recipient chromosome Gene conferring StrR Double-stranded DNA binds to the surface of a competent cell. 2 Single strand enters the cell; the other strand is degraded. 3 The strand integrates into the recipient cell's genome by homologous recombination. The strand it replaced will be degraded. Streptomycin-sensitive daughter cell Streptomycin-resistant daughter cell 4 After replicating the DNA, the cell divides. Non-transformed cells (Str) die on streptomycin-containing medium whereas transformed cells (Str) can multiply

Explanation / Answer

This picture explains how bacteria achieve drug resistance. A gene conferring resistance to streptomycin is transferred by a process called transformation. The bacteria which inherit this gene become resistant to the drug.

How do the gene enter the bacteria? It is a random chance event.

How does the gene integrate with the bacterial genome? The gene may also have nucleases which cleave the bacterial genome at the desired locations and insert itself in the genome.

I am most interested in the way the gene integrate with the bacterial genome.

Got confused when found not all bacteria inherit the drug resistance gene. Do you know why? The gene is present on (outer) one of the 2 stands of the circular chromosome of the bacteria. When bacteria divide by binary fission, the inner strand which may be used as template for chromosome multiplication is not having drug resistance gene, so one of its progeny do not inherit it.

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