What is the purpose of including a PCR reaction with no added DNA? Isn’t this ju
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What is the purpose of including a PCR reaction with no added DNA? Isn’t this just a waste of expensive enzyme when we know that without template, you will not get any PCR products? What is the purpose of including a PCR reaction with no added DNA? Isn’t this just a waste of expensive enzyme when we know that without template, you will not get any PCR products? What is the purpose of including a PCR reaction with no added DNA? Isn’t this just a waste of expensive enzyme when we know that without template, you will not get any PCR products?Explanation / Answer
PCR reaction without a DNA template acts as a negative control for the experiment. A negative control helps to rule out any false positive results during amplification. If any amplication is observed in the negative control then it means that the experiment is flawed and the composition of the PCR reaction mixture has to be checked for possible contamination.
PCR reaction without a DNA template can also be used to check the formation of primer dimer of forward and reverse primer.
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