2. (2pts each) Consider cargo proteins that are destined for secretion to the ex

Question

2. (2pts each) Consider cargo proteins that are destined for secretion to the extracellular space. Please predict where the cargo will end up (ex. in the cytoplasm, a specific organelle, transport vesicles, multiple places, extracellular space) for: (i) & () Each individual condition; & (ii) The combination of both conditions A. () Normal Sar1 is mutated so it binds tightly to GDP and cannot exchange it for GTP. (This leads to Sar1 -GDP being the only form of the protein in the cell.) (i) COPII coat proteins are mutated so they bind both to active Sar1 and active ARF1 (ii) Both B. () A KDEL signal sequence is added to the C-terminus of the cargo protein. (i) The signal recognition particle is mutated so it can't recognize ER signal sequences. (ii) Both C. () V-SNAREs on COPII coated vesicles are mutated to be unable to bind t-SNAREs (ii) A transmembrane domain is added to the cargo protein (ii) Both D. () Receptors in the Golgi are mutated so they are unable to bind to cargo proteins. (i) COPI coat proteins are mutated so they are unable to bind to membranes. (ii) Both

Explanation / Answer

A) i - cytosolic side of the endoplasmic reticulum

ii- Golgi

iii - ER

B) i-ER

ii - Golgi

iii - Golgi

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