21. Which of the following is NOT true of a DNA probe? A. A single-stranded DNA

Question

21. Which of the following is NOT true of a DNA probe? A. A single-stranded DNA fragment that is complementary to part of the gene of interest B. Can be used to identify different bacterial species in the environment C. Must be labeled with a molecular tag or beacon D. Used to screen a genomic library for a particular gene E. All of the above are true 22. Which of the following techniques is used to separate DNA fragments based on size? A. Agarose gel electrophoresis B. Fluorescent in situ hybridization (FISH) C. Microarray D. Polyacrylamide gel electrophoresis (PAGE) E. Southen blot 23. The distance of migration through the gel is inversely correlated to the size of the DNA fragment. A. True B. False 24. Which of the following processes uses distinct banding patterns to differentiate DNA samples after restriction digestion? A. Agarose gel electrophoresis B. Fluorescent in situ hybridization (FISH) C. Microarray D. Restriction fragment length polymorphism (RFLP) E. Southern blot 25. Which of the following methods is typically used to detect the amount of mRNA? A. Colony blot B. DNA fingerprinting C. Microarray D. Northern blot E. Southerm blot 26. Which of the following techniques is used to amplify a specific DNA fragment? A. Agarose gel electrophoresis B. DNA sequencing C. Microarray D. Polymerase chain reaction (PCR) E. Restriction enzyme digestion 27. PCR occurs over multiple cycles, each containing three steps: denaturation, annealing, and extension.

Explanation / Answer

21 - The correct answer is option E that is all of the above statements nts are correct for the probe

22 - The correct answer is option A that is agarose gel electrophoresis. It is used to separate DNA fragments of different size.

23 - A) False.

It is not inversely proportional. Smaller the fragment faster it travels.

24 - The correct answer is option D that is RFLP.

25- The correct answer is option D that is northern blotting

26 - The correct answer is option D that is Polymerase chain reaction.

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