From the next-to-the-last dilution blank, plate (in triplicate) 0.1mL If the col

Question

From the next-to-the-last dilution blank, plate (in triplicate) 0.1mL If the colony counts the following day were 121, 134, and 122, what is the density of bacteria in the original sample? (4 points) avg: 12sua 7 1.25?7x107 (linK?) 7. Diagram how you would do the following dilutions &/or calculations(@ 2pts) A 1:20 dilution of contaminated ground beef if you only had 20g to start with: . .A series of 1:2 dilutions of patient serum starting at "undiluted" in the first well of a 96-well microtiter plate: Diluting lysozyme from 20mg/mL to 4 ug/uL: 20 ual mt_5 . Diluting a "5X" PCR buffer to "1X" in a total volume of 25ul: Calculate pfu/mL of a phage stock if you counted 31 plaques on a plated dilution of 105 plate: Make a 1:6 dilution of Kovac's reagent:

Explanation / Answer

Answer:

7)

a) 1:20 dilution of contaminated ground beef if you only had 20g to start with:

b) A series of 1:2 dilutions of patient serum starting at "undiluted" in the first well of a 96-well microtiter plate:

c) Diluting lysozyme from 20mg/ml to 4ug/ul

d) Diluting a "5X" PCR buffer to "1X" in a total volume of 25ul.

e) Calculate pfu/mL of a phage stock if you counted 31 plaques on a plated dilution of 10-5 plate:

f) Make 1:6 dilution of Kovac's reagent:

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