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Use the following data to answer the questions 1 and 2. In a sample of double-st

ID: 25688 • Letter: U

Question

Use the following data to answer the questions 1 and 2. In a sample of double-stranded DNA from a particular bacterium with a circular chromosome, 43% of the nucleotide bases are Guanine (G). Sodium hydroxide (NaOH) "denatures" DNA by disrupting the hydrogen bonds and causing the strands to separate. You can separate one strand from the other by gel electrophoresis; one strand runs faster in the gel than the other strand. You find that the "faster" strand has 62% G.

1. What percentage of nucleotide bases in the original double-stranded molecule are A?
a. 7%
b. 14%
c. 43%
d. 57%
e. Cannot be determined from the information provided.

2. What percentage of nucleotide bases in the faster single-strand are C?
a. 0%
b. 7%
c. 24%
d. 38%
e. Cannot be determined from the information provided.

EXPLAIN YOUR ANSWER PLEASE!! :D

Explanation / Answer

Chargaff's Rule A=T G=C 1. a 2. c When you split the strands, keep in mind that the 43% original equals 86% of the total 200% created. 86-62=24

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