can someone help with with part b with identifying the number associated with as
ID: 257004 • Letter: C
Question
can someone help with with part b with identifying the number associated with assays
1. (6 similar to what Jacob a pts) You are analyzing mutants in an Operon in an uncharacterized bacterial species using a method nd Monod did to analyze the lac operon (see your text book Fig 14.7) f Sucrose to produce a sucrose metabolizing enzyme, the sing a reporter assay. A pink colored product is produced when This operon is induced in the presence o expression of which can be monitored u an artificial substrate is added and the sucrose metabolizing enzyme is present. Like t this assay is quantitative so the more enzyme, the darker the pink. he ONPG assay In this operon, induction by sucrose is mediated by an Activator protein that when bound to its inducer sucrose, is able to bind to Operator A. The operon is also repressible by lactose. Repression by lactose is mediated by a Repressor protein that when bound to its co-repressor lactose, is able to bind to Operator B. (2 pts) Based on this description, state whether the activator, the repressor, both, or neither would be bound to their respective operators under each set of conditions in wild type bacteria. a. No Lactose No Sucrose Lactose No Sucrose No Lactose Sucrose Lactose Sucrose Wild Type | Ne.eter | Reprreso . | Aellelor- ?Both A series of mutants is described below Activator Mutant A- Activator cannot bind to NA Activator Mutant B- Activator cannot bind to sucrose Repressor Mutant A-Repressor cannot bind to DNA Repressor Mutant B- Repressor cannot bind to lactose . b. (4 pts) Pink color was measured in wild type bacteria under the listed conditions. Based on the measurements in the wild type condition, state the amount of pink color that would be produced.Explanation / Answer
-lactose –sucrose
+lactose –sucrose
-lactose +sucrose
+lactose +sucrose
Wild type
0.030
0.030
1.891
0.124
Activator mutant A
0.030
0.030
0.030
0.030
Activator mutant B
0.030
0.030
0.030
0.030
Repressor mutant A
0.030
0.030
1.891
1.891
Repressor mutant B
0.030
0.030
1.891
1.891
-lactose –sucrose
+lactose –sucrose
-lactose +sucrose
+lactose +sucrose
Wild type
0.030
0.030
1.891
0.124
Activator mutant A
0.030
0.030
0.030
0.030
Activator mutant B
0.030
0.030
0.030
0.030
Repressor mutant A
0.030
0.030
1.891
1.891
Repressor mutant B
0.030
0.030
1.891
1.891
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