What is the correct answer to this question and why? Map Sapling Learning macmil

Question

What is the correct answer to this question and why?  

Map Sapling Learning macmillan learning An experiment was conducted to determine the effects of a novel promoter on the transcription of a gene The cloned promoter and coding regions were ligated to luciferase, a reporter gene. Several regions of DNA in the promoter region were deleted. Each construct was transformed into cells, and the luciferase activity was measured. The data from the experiment is listed in the table below DNA Region Deleted| Relative Luciferase Expression No Deletions 100 320 100 15 Which conclusions can be drawn from the data? Enhancers that increase transcription are located in region A 1 ranscription binding sites are not found in region B Region D does not contain any response elements Basal promoters are located in region D because gene expression decreases when it is deleted Transcription decreases when region D is removed because the promoter is not located in region D

Explanation / Answer

From the given data , we can infer that region B has no effect on transcription.

Region A, must be a repressor. As it is deleted, transcription has increased.

Region C as well as D, contain enhancers. When these are deleted, transcription goes down.

However, on deleting D , transcription is low by less than 1% of normal levels. This is possible when basal promoter elements are removed.

Thus, correct option should be:

(iv) basal promoters are located in region D, because gene expression decreases when it is deleted.

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