We have four tubes containing individual primers that are designed to amplify tw

Question

We have four tubes containing individual primers that are designed to amplify two particular regions of the human genome. While organizing tubes, we accidently erased all the information regarding these tubes. Use the following web tools to figure out which primer is paired with which another to amplify which region of human genome. (UCSC In-Silico PCR: http://genome.ucsc.edu/cgi-bin/hgPcr?hgsid=568061131_Us1GaoCaBcZKgNTGBAA8MZeDDOz3) Primer 1: 5’-GTTTGGAGACAACGCTGGCC-3’ Primer 2: 5’-CTGCCCAGGGCCTCACCACCA-3’ Primer 3: 5’-ACATTTGCTTCTGACACAAC-3’ Primer 4: 5’-AATTGGAAGCAAATGACATC-3’ Once you find out the genomic regions, explain what size of each PCR product you will obtain if you actually do PCR? Also, describe what kind regions of these two PCR products are coming from (such as intron or exon).

Explanation / Answer

It wil give 175 bp product by using primer 1and 3

On chromosome 7 that will contain intron and exon both.

If we use primer 2 and 3 it will give 142 base pair on chromosome 11 having exon region.

Related Questions

Navigate

• Browse (All)
• Browse W
• Subjects

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.