10) DNA and protein isolation techniques: During the miniprep experiment (to iso

Question

10) DNA and protein isolation techniques: During the miniprep experiment (to isolate DNA) we lysed open bacteria in a solution containing EDTA, a chelator. When we isolated DNA for our PCR reaction, we broke open the chelator protect DNA from? 9 t ? s we tewn to runne cells in the presence of another chelator (instagene matrix), What does a R reaction, our DNA template shouldn't have any chelators present so we had to spin away the instagene matrix and sonly use DNA in the supernatant. What reagent in a PCR reaction relies on metal cofactors to function? f4 ?.?) In our PCR reaction, we looked at differences in gene length for a region of chromosomal DNA. To get results and determine the size of the DNA that was generated by the PCR reaction, we loaded our samples into and measured the length of DNA by comparing how far it migrated to iiIf our PCR reaction had worked and we saw two bands, what would that tell us? d) When we broke open the bacteria to isolate the GFP protein, we digested the tough outter layer of bacteria with an enzyme, and then placed them in the freezer i) Why does placing the bacteria in the freezer cause them to break open (and release their protein contents)? Slaw oun thety ???

Explanation / Answer

ii. if our PCR product shows two bands then it shows polymorphism of gene/DNA/targeted sequence.

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