Q1. A single ATP synthase is capable of generating about 30 ATP molecules for ev

Question

Q1.

A single ATP synthase is capable of generating about 30 ATP molecules for every 100 protons that flow through its F0 complex. If we isolated a mutant ATP synthase in which one of its three beta-subunits could not bind ADP, how many ATP molecules would this mutant be expected to generate for every 100 protons that flow through it?

Select ONE option:

1. 30 ATP molecules

2. 20 ATP molecules

3. 10 ATP molecules

4. 0 ATP molecules

Q2.

The release of ATP by the beta subunit is most directly dependent on:

Select ONE option:

1. the flow of protons from the intermembrane space to the matrix.

2. the rotation of the c-ring in the membrane.

3. the passage of electrons along the electron transport chain.

4. a conformational change in the beta subunit.

Q3.

What defines the direction of rotation of the c-ring in the F0 complex?

(Select ALL that apply.)

1. Amino acids side chains in c-subunits will repel a bound proton if the c-ring rotates in the wrong direction

2. The availability of ADP and inorganic phosphate as substrates

3. The direction of the proton gradient relative to the inner mitochondrial membrane

4. The conformational changes in the alpha and beta subunits

Q4.

The release of protons by the c-ring is more favorable on the matrix side of the membrane because:

Select ONE option:

1. of the lower concentration of protons on the matrix side.

2. of the rotation of the c-ring.

3. ATP is synthesized on the matrix side.

4. the citric acid cycle enzymes are on the matrix side.

Q5.

Select the correct location of the following within the cell:

A. Glycolysis:

Select ONE option:

1. Mitochondrial matrix

2. Inner membrane

3. Inner membrane space

4. Outer membrane

5. Cytoplasm

B. Electron Transport Chain complexes:

Select ONE option:

1. Mitochondrial matrix

2. Inner membrane

3. Inner membrane space

4. Outer membrane

5. Cytoplasm

C. ATP synthase:

Select ONE option:

1. Mitochondrial matrix

2. Inner membrane

3. Inner membrane space

4. Outer membrane

5. Cytoplasm

D. Complex 1:

Select ONE option:

1. Mitochondrial matrix

2. Inner membrane

3. Inner membrane space

4. Outer membrane

5. Cytoplasm

E. Highest proton concentration:

Select ONE option:

1. Mitochondrial matrix

2. Inner membrane

3. Inner membrane space

4. Outer membrane

5. Cytoplasm

Q6.

Part 1. Select the metabolic process most closely related to the following starting reactants

A. Carbon dioxide (CO2):

Select ONE option:

1. Glycolysis

2. TCA cycle

3. Electron Transport Chain

4. Photosynthesis

B. Glucose (C6H12O6):

Select ONE option:

1. Glycolysis

2. TCA cycle

3. Electron Transport Chain

4. Photosynthesis

C. Acetyl-CoA:

Select ONE option:

1. Glycolysis

2. TCA cycle

3. Electron Transport Chain

4. Photosynthesis

D. NADH:

Select ONE option:

1. Glycolysis

2. TCA cycle

3. Electron Transport Chain

4. Photosynthesis

Part 2. Select the metabolic process most closely related to the following products.

A. Carbon dioxide (CO2):

Select ONE option:

1. Glycolysis

2. TCA cycle

3. Electron Transport Chain

4. Photosynthesis

B. Glucose (C6H12O6):

Select ONE option:

1. Glycolysis

2. TCA cycle

3. Electron Transport Chain

4. Photosynthesis

C. Pyruvate:

Select ONE option:

1. Glycolysis

2. TCA cycle

3. Electron Transport Chain

4. Photosynthesis

D.Water (H2O):

Select ONE option:

1. Glycolysis

2. TCA cycle

3. Electron Transport Chain

4. Photosynthesis

Q7.

By which of the following mechanisms could ATP synthesis potentially be inhibited?

(Select ALL that apply.)

A. By dissociating the F1 complex from the F0 complex

B. By blocking proton passage through the F0 complex

C. By blocking the rotation of the ?3?3 hexamer of the F1 complex

D. By blocking inorganic phosphate from binding to the F1 complex

Q8.

What would happen to the mitochondrial proton gradient, mitochondrial respiration (mitochondrial oxygen consumption), and the NADH oxidation rate in cells treated with carbonyl cyanide-4-trifluoromethoxyphenylhydrazone (FCCP), a molecule that increases the permeability of the inner mitochondrial membrane to protons?

Part 1. The mitochondrial proton gradient would:

Select ONE option:

1. increase

2. decrease

Part 2. Mitochondrial respiration (mitochondrial oxygen consumption) would:

Select ONE option:

1. increase

2. decrease

Part 3. The NADH oxidation rate would:

Select ONE option:

1. increase

2. decrease

.

Explanation / Answer

2) Answer A:

the flow of protons from the intermembrane space to the matrix.( ATP synthesis are coupled by a proton gradient across the inner mitochondrial membrane)

5) A) glycolysis occurs in the cytoplasm of all cells

B) electron transport chain is found in the inner mitochondrial membrane

C) In mitochondria, ATP synthase is located in the inner membrane

D) Complex 1 occurs in the inner mitochondrial membrane

E) Highest proton concentration occurs in the inner membrane space

6) A) starting reactants of photosynthesis is CO2

B) Glycolysis

C) TCA cycle or Krebs cycle

D) Electron Transport Chain

Part2:

A) TCA cycle or Krebs cycle

B) Photosynthesis

C) Glycolysis

D) Electron transport chain

4) Answer 2:

the movement of protons through the half channels from the high proton concentration of the inner membrane space to the low proton concentration of the matrix powers the rotation of the c ring

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