a. predict the sizes of the PCR bands amplified from the wild type and CCR5 32 a

Question

a. predict the sizes of the PCR bands amplified from the wild type and CCR5 32 alleles of the CCR5 locus when using the appropriate primers (see sequence above)

b. Based on the results, what genotypes were detected within the class population?

c. Was this an expected result based on the predicted frequencies of delta 32 CCRS within the general population (Based on Western European populations), i.e~1% homozygous mutant, ~10% hetsrozygote)
b.

Tosid you in your decision, the relevent portions ofthe codrg uence for and CCRS 32 are provided in the dagram below. Because the indicated sequerces are too long to ft onto one line, they have been split up across three ines. The CORS sequence is shown in uppercase white the COSS2 sniumce is shown in bwrenn. The sniurees algned against one another, and the numbers at the top indicate the positions of the bases within the erce. The biu" region nicates the regn that deleted in the cosu2 mutation. Stes where the availeble primers are able to bind have been underlined and designated as elte "A OCKS Line 1 .aceeaccesscacessgaga atggtetagagesett 3 (CCRSA32 iin. 3) gtear 3

Explanation / Answer

a. the primer for wild type extends from 583rd base to 742 base, so the amplicon size will be 742-583= 159bp

the primer for CCR5 delta 32 extends from 583rd base to 710th base, so expected amplicon size is (710-583= 127). There is a deletion of 32 bases from this region so the actual amplicon size will be 127-32= 95 bp

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