An mRNA contains the following sequence: 5’-CAUGAACUGCCUGACCCACACUUAUCACUAAGUAGC

Question

An mRNA contains the following sequence:

5’-CAUGAACUGCCUGACCCACACUUAUCACUAAGUAGCCUAGCAGUCUGA-3’

which translates into polypeptide as:

N-Met-Asn-Cys-Leu-Thr-His-Thr-Tyr-His-C

The following three mutant proteins are formed because of mutations in the original gene sequence that encodes the mRNA above. Identify what mutations caused each of these altered polypeptide sequences.

N-Met-Asn-Cys-Leu-Thr-His-Thr-C

N-Met-Asn-Cys-Leu-Thr-His-Thr-Tyr-His-Lys-C

N-Met-Asn-Cys-Leu-Thr-His-Thr-Tyr-His-Tyr-Ser-Ser-Leu-Ala-Val-C

Explanation / Answer

1. Deletion is the mutant

2 and 3 are insertion since the length of the protein has increased

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