The terrigen cloud has enclosed the New York Metropolitan area, activating the i

Question

The terrigen cloud has enclosed the New York Metropolitan area, activating the inhuman genome via the transcription factor IG. A BIO3320 friend of yours is not inhuman, but really wants some superpowers. For an independent study, they isolated a small amount of IG and determined that it can make mice perform better on every behavioral tests they could think of. To produce IG for personal use, your friend obtained a complete genomic clone of the IG gene, connected it to a strong promoter and introduced the plasmid into E. Coli cell. You find your friend crying in the lab, devastated to find that no IG was produced in the cells. What is the likely explanation for this failure?

1) Your friend should have started with an IG cDNA instead of a genomic clone.

2) Eukaryotic IG transcript is targeted for destruction by E. Coli miRNAs

3) Your friend is an over expression mutant for the dumbass protein

4) Eukaryotic proteins cannot be produced by an E.Coli cell

5) Sigma promoter cannot bind to a eukaryotic TATA box

Explanation / Answer

Answer: Choice 4

Reason: An eukaryotic protein requires a strong promoter as well as splicing out of the intronic sequences from the exons. This process ensures generation of a functinal mRNA transcript and hence a functional polypeptide from it. This polypeptide then undergoes post-translational modification in order to generate a 3-dimensional functional protein. Since a bacterial cell lacks alterntive splicing as well as post-translational modification mechanisms, an eukaryotic gene can generally be not expressed in a prokaryotic host.

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