The terrigen cloud has enclosed the New York Metropolitan area, activating the i

Question

The terrigen cloud has enclosed the New York Metropolitan area, activating the inhuman genome via the transcription factor IG. A BIO3320 friend of yours is not inhuman, but really wants som they isolated a small amount of IG and determined that it can make mice perform better on every behavioral tests they could think of. To produce IG for personal use, your friend obtained a complete genomic clone of the IG gene,connected it to a strong promoter and introduced the plasmid into E. Colicell You find your friend crying in the lab, devastated to find that no IG was produced in the cells. What is the likely explanation for this failure? e superpowers. For an independent study, O Eukaryotic proteins cannot be produced by an E.Coli cell Eukaryotic IG transcript is targeted for destruction by E. Coli miRNAs Your friend should have started with an IG cDNA instead of a genomic clone Sigma promoter cannot bind to a eukaryotic TATA box Your friend is an over expression mutant for the dumbass protein

Explanation / Answer

According to me answer should be: 4

(Sigma promoter cannot bind to a eukaryotic TATA box)

The transcription process in E.coli and eukaryotes are different in terms of the structures of promoters and the complexity of RNA polymerases and transcription factors. E.coli has one RNA polymerase which interacts with different sigma factors and which is responsible for the specificity of transcription initiation. But, Transcription in eukaryotes is mediated by three highly RNA polymerases with a complex subunit structure, require the interaction of different transcription factors for transcription initiation. Eukaryotic promoters have a highly modular structure and typically contain a TATA box, an initiator element around the transcription initiation site which can confers the tissue and cell specificity. Sigma factors recognizes the promoters of the genes in its group, not by other sigma factors.

Related Questions

Navigate

• Browse (All)
• Browse T
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.