Order the best steps to take to identify the gene and the mutation that causes l

ID: 261216 • Letter: O

Question

Order the best steps to take to identify the gene and the mutation that causes lung cancer

Not all answers will be placed:

(1.) Isolate the tumor .

Options:

Generate a cell line from the tumor . l

ightly fragment the genome .

sequence the tumor-cell genome .

isolate transformed colonies .

transfect genome into mouse cell line .

determine shared transfected region .

repeat the small fragments and sequence the gene .

generate transgenic mice with same mutation .

(6) Introduce the mutation in cell Culture (Pre-selected answer)

The best steps to take to identify the gene and the mutation that causes lung cancer

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