Order the steps of generating a yeast knockout. already given: identify sequence

Question

Order the steps of generating a yeast knockout.

already given: identify sequence of target gene

already given: verify absence

put into order in between:

-use PCR to generate a DNA fragment that contains a selectable marker flanked by the target gene sequence at the 3' and 5' ends

-induce cells to take up the PCR product

-homologous recombination incorporates the PCR insert in place of the target gene

-Verify the PCR insert was not inserted randomly into the genome

-select for the cells that contain the selectable marker

Explanation / Answer

the steps in order would be as follows:

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