Hi, id really appreciate it if you ca please help me with this question. PLease

Question

Hi, id really appreciate it if you ca please help me with this question.

PLease answer these questions.

2A – What is the purpose of using the 3 different agars (TH, Gr, Bl)? How do the clinical isolates compare to the wildtype strains?

2B – Explain the regulatory network shown in this part of the figure.

2C – Which genes appear to be most important for pigment production?

. List at least 3 characteristic/X D lit article #4.pdf xy D lit article#4.pdf xMolecular Characterization of x Person 2 ? c Secure l https://mycourses.hìghpoint.edu/bbcswebdav/pid-7491 99-dt-content-rid-13970035-/courses/58116/lit%20article%20%234.pdf ? cyl mutants cyl + abx1 mutants Abx1 CCH1318 CCH1319 CCH1320 CCH1321 CCH1322 CCH1323 CCH1346 abx1 mutants CCH1326 CCH1327 CCH132 cylD acpC cylA CCH1330 CCH1331 CCH1333 CCH1334 CCH1337 CCH1338 CCH1339 CCH1340 CCH1341 cylX cylG cylZ cylB Unknown CCH1348 Wild-type strains CCH1342 CCH1343 CCH1344 CCH1345 CCH1347 4 NEM316 BM110 515 3 CCH1352 CCH1084 analysis, organized according to the identified mutation, and of GBS wild-type strains NEM316, BM110, 2603VR, 515, H36B and A909. TH, Todd-Hewitt agar; Gr, Granada agar, B Columbia agar supplemented with 5% horse blood. B Structure and regulation ofthe loperon. Red crossed line, tra scriptional repression; green straight arrow, activation; black bent arrow, initiation of transcription. (C) Distribution of identified mutations in the cyl operon and in the cyl regulator gene abx1 among 31 GBS strains; note that no mutation was associated to the NH/NP phenotype among 3 sequenced strains. Questions for Lit #4.docx Study Guide#4 s20.-.docx E REVISED Chpt16 17 .pptx ? Chpt16-s2018.pptx Chapt15-s2018.pptx Show All

Explanation / Answer

In microbiology, Granada Medium (Gr agar) is selective culture medium to isolate and identify beta-hemolytic Group B Streptococcus species (S. agalactiae). Positive colonies (S. agalactiae, wild type) give specific red pigment due to accumulation of polyenic pigment. However, negative species (other species) give white colour colony. In contrast, Todd Hewitt (TH) media Broth is being used to identify Group A streptococci. Columbia blood agar (BI) isolation and growth of a variety of fastidious microorganisms.

abx1 negatively interact with histidine kinase CovS. CovS predominantly regulate the activity of CovR (which is under control of Skt1). CoVS is required for the transcriptional activation of Cyl-operon.

CYL gene are major regulator of pigment formation. Additionally, CovR and ABX1 are also ket transcriptional regulator of pigment formation because they control the expression of CYL genea or CYL-operon.

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