13 Peaks 2. tamicd 13 L-Methionine6. Using the elution i4 3. Lerin e 4. Ghycine

Question

13 Peaks 2. tamicd 13 L-Methionine6. Using the elution i4 3. Lerin e 4. Ghycine 5. L-Hstidin e L?Systei nine profile to the left for a 11 12,3 16-~ 17 16 di1hydrolyzed peptide 17 L-Phenylalanine · L-Arginine 7. L-Threonine 18 LLysin e 8· L-Alanine 9. L-P roline 10. Ammonium 11·L-Tyrosine 100 followed by a reaction with flourescamine, how big is your peptide? A) 5 residues B) 6 residues S0 0.0 1.0 2.0 3.0 min 200 100 D) 8 residues E) None of these. 0.0 1.0 2.0 min 7. Using the above elution profile which of the following represents the amino acids in vour peptide'? A) E,EG,N,T B) C,D,L,W,Y C) A,F,G,M,V DE,E,M,SY E) D,I,M,S,Q

Explanation / Answer

D) The elution profile, based on the chromatogram in which the Y-axis represents the mAu (milli absorbance unit) and X-axis represents the retention time. mAu indicates the absorption of analytes and is used to determine the concentration of analytes. Retention time is the time between the injection of the analyte to the detection of the analyte. By comparing and observing the retention time of the amino acids from both the graph, we can interpret the results. Here, the amino acids which are represented by the peptide are Glutamic acid (E), Serine (S), Tyrosine (Y), Methionine (M) and Phenylalanine (F). Thus, the option D satisfies the answer to the question.  

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