You are given a short fragment of DNA to sequence. You set-up a series of four d

ID: 262272 • Letter: Y

Question

You are given a short fragment of DNA to sequence. You set-up a series of four dideoxy reactions, and then separate the products of the complementary strands that you produced by gel electrophoresis and obtain the following banding pattern:

5’ ______________________________ 3’

What would have been the sequence of the template DNA (label the 5’ and 3’ ends):

________________________________

Reaction Containing ddATP ddTTP ddCTP ddGTP Origin Write-out the sequence of the DNA synthesized complementary to the template DNA:

Explanation / Answer

The order of sequence is from bottom to top.
The sequence is:
5' TACTGATAGC 3'

The sequence of template strand is:

3' ATGACTATCG 5'

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You are given a short fragment of DNA to sequence. You set-up a series of four d

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Explanation / Answer

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