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7. In the lab, you are investigating a newly identified virus to determine how i

ID: 262490 • Letter: 7

Question

7. In the lab, you are investigating a newly identified virus to determine how it infects host cells. Luckily, you obtained a GFP-expressing virus from a colleague, so you can observe infection via fluorescent microscopy. You decide to use this tool to test whether clathrin-dependent endocytosis is the required entry mechanism for this virus.

A. (4pts) Please describe a general experimental design to test this hypothesis through the use of genetic mutation, including the effect(s) of the protein mutation(s) you would introduce to the host cell.

B. (4pts) Consider the anticipated results of this experiment. What are two results you may observe. What would each of these results suggest about how the virus enters the cell?  

Explanation / Answer

1.

b)

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