In a sample of four humans (1-4), genomic DNA was isolated and subjected to the

Question

In a sample of four humans (1-4), genomic DNA was isolated and subjected to the PCR you designed above. The resulting PCR products were separated by gel electrophoresis, and a representation of the results is shown below in the lanes labeled 1-4.
e. Samples 1 and 2 are from a husband and wife, however they got mixed in the laboratory so that it was not possible to determine which sample belonged to which individual. Without re-doing the PCR, which sample belongs to which individual? Briefly explain your answer. f. Samples 3 and 4 are children of the parents whose profiles are shown in lanes 1 and 2. If one child is male and one is female, which is which? Briefly explain your answer. g. When equal amounts of cDNA from individuals 1 and 3 were used as the template for PCR, I observed the results shown in the last two lanes of the gel, where the amount of product from individual 3 was reduced compared to that from individual 1. Explain why we see this result for individual 3. Please include in your answer which of individuals 1-4 have hemophilia, and how the Alu element might cause the disease.

Explanation / Answer

In a sample of four humans (1-4), genomic DNA was isolated and subjected to the

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