You are studying the EGFR signaling pathway in a tumor cell line isolated from a

Question

You are studying the EGFR signaling pathway in a tumor cell line isolated from a melanoma. These cells have uncontrolled proliferation. They continue to grow in the absence of EGF or any other growth factor. Another group determined this was due to constitutive activation of the Ras-MAP kinases pathway.

A. You compare the levels of EGFR to wild type cells and see no difference. How would you experiementally compare EGFR levels? What could account for this difference in proliferation but not in overall receptor protein levels?

B. You hypothesize that the Raf kinase may be constitutively active. You are given purified MEK protein. How could you test the state of kinase activation in these cells?

C. You hypothsize that either Ras or MAPK kinase must be constitutively active. Design experiements to prove which hypothesis is correct.

Explanation / Answer

You are studying the EGFR signaling pathway in a tumor cell line isolated from a

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