3. (6 points) Below figure shows three temperature-sensitive (ts) yeast mutants,

Question

3. (6 points) Below figure shows three temperature-sensitive (ts) yeast mutants, Mut-A, Mut-B, and Mut-C. Each yeast culture was first grown at permissive temperature (22-C) and then the temperature was shifted to restrictive level (36-C) Mut-A Two cell cycles 22°C-. 36°C Cells arrest and display various states of cell Mut-B Two cell cycles 22°C ? 36°C Permissiverestrictive Cells arrest without buds Mut-C Two cell cycles 22°C.+ 36°C Cells arrest with buds For each mutant identify: - Which mutants were cell-division-cycle (cdc) mutants: At what point of cell cycle the arrest took place: If cells appeared to be synchronized, or not, during cell division after temperature shifted back to permissive temperature:

Explanation / Answer

3. (6 points) Below figure shows three temperature-sensitive (ts) yeast mutants,

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