Component Tube 1 [Unknown] Tube 2 [Unknown] uL Lipid Sample 25 uL 100 uL uL Isop

Question

Component

Tube 1 [Unknown]

Tube 2 [Unknown]

uL Lipid Sample

25 uL

100 uL

uL Isopropanol

75 uL

0 uL

Using y equation from the Standard Curve, I calculated the Mass of the Unknown cholesterol present in tubes 1-2 by plugging in absorbance

Tube

A520

Mass of Cholesterol (ug) [Unknown]

1

0.075

4.37

2

0.090

5.59

QUETIONS:

1. Based on the volume of lipid sample added to each tube 1-2, determine the concentration of cholesterol (mg/mL) in your lipid sample in isopropanol.

2. Based on the volume of lipid sample 150 uL, determine the concentration of cholesterol (mg/mL) present in the original lipid sample.

3. Using the molecular weight of cholesterol (386.7 g/mol), calculate the concentration of cholesterol (mM) in the entire membrane sample.

Component

Tube 1 [Unknown]

Tube 2 [Unknown]

uL Lipid Sample

25 uL

100 uL

uL Isopropanol

75 uL

0 uL

Explanation / Answer

Component Tube 1 [Unknown] Tube 2 [Unknown] uL Lipid Sample 25 uL 100 uL uL Isop

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