A number of temperature-sensitive mutants of budding yeast that block the cell c

Question

A number of temperature-sensitive mutants of budding yeast that block the cell cycle at the G1/S boundary were recovered. They were crossed in all pairwise combinations and the diploids were tested for the ability to grow at 37¡C. The results are given below. How many genes are represented among these 5 mutants?

  a b             c              d             e

                a             –             –             +             –             +

                b                             –             +             –             +

                c                                              –             +             –

                d                                                             –             +

                e                                                                              –

Explanation / Answer

When two mutants are crossed and the resultant F1 progeny shows WT phenotype, it means that both the mutations are located in different genes. i.e. they are non-allelic.

When two mutants are crossed and the resultant F1 progeny shows mutant phenotype, it means that both the mutations are located in the same gene. i.e. they are allelic to each other.

'+' means WT phenotype

'-' means mutant phenotype

Complementation groups:

i. a = b = d

ii. c = e

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