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4. The gal operon in E. coli encodes galactose utilization enzymes that allow th

ID: 268153 • Letter: 4

Question

4. The gal operon in E. coli encodes galactose utilization enzymes that allow the cell to metabolize galactose. Expression of the gal operon includes regulation by the CAP (CRP) protein, in common with the lac operon. What can you assume is the funetion of CAP and in what way will it be used to regulate the gal operon? 4 pts 5. Which of the following activities would you not associate with a prokaryotic transcriptional activator? 2 pts a. Stabilization of RNA pol binding b. Facilitating transition of RNA pol to open complex c. recruitment of chromatin modifiers d. binding of sigma to the promoter

Explanation / Answer

Q4:- Explanation

The CAP ( Catabolite Activator protein ) or CRP binds to the promoter to facilitate gene expression both in lac and gal operon. In galactose operon two promoters are present binding on promoter P2 promotes gene expression while binding at P2 inhibit expression.

Q5- Option C

Explanation:- Recruitment of chromatin modifiers is not function of transcription activators in prokaryotes.

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