why did you digest the plasmid with XhoI? What is your expectation if the plasmi
ID: 268211 • Letter: W
Question
why did you digest the plasmid with XhoI? What is your expectation if the plasmid is a reclosed vector without an insert? What is your expectation if your plasmid has an insert (how many bands, what are their sizes)?
You have then digested your plasmids with XhoI, AlwNI and both enzymes.
Paste the photograph of your gel (see p. 105) below:
why did you digest the plasmid with XhoI? What is your expectation if the plasmid is a reclosed vector without an insert? What is your expectation if your plasmid has an insert (how many bands, what are their sizes)?
12 3 45 6 7 Bio-Rad LaboratoriesExplanation / Answer
Please find the answers below:
According to the information the plasmid containing gene of interest has been digested with Xhol and allowed to resolve on gel. The banding patterns has been noticed and following answers could be deduced from it:
why did you digest the plasmid with XhoI?
Answer: In order to ascertain the total size of the plasmid after insertion of the gene of interest, it is necessary to digest the plasmid with a restriction endonuclease enzyme, Xhol in this case.
What is your expectation if the plasmid is a reclosed vector without an insert?
Answer: If the plasmid is re-closed without an insert, the size of the band will be same to that of negative control plasmid. This suggests that the size of native plasmid and potentially re-constructed plasmid would be same.
What is your expectation if your plasmid has an insert (how many bands, what are their sizes)?
Answer: If the plasmid has the insert, it would be digested at two locations, each flanking the gene of interest. Thus, the two size bands so produced would be one corresponding to the native un-constructed plasmid and other similar to the gene on interest.
Related Questions
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.