Bio-123 Molecular Biology Part 2 Post Lab s a restriction map for the plasmid pG

Question

Bio-123 Molecular Biology Part 2 Post Lab s a restriction map for the plasmid pGEN 101 (total length 20 Kb). Using this map as a eve the number of restriction fragments along with their associated lengths that would guide, give result from EooRl and Baeing PGEN 101 with the restiction enzymes EcoRi, BamHl and a combination of BamH 17 Kb 32 Kb EcoRi BamHl PGEN 101 87 Kb 5.5 Kb 9 Kb EcoRI ECORI Digest performed Size of the fragments obtained (kb) EcoRI BamH Double Digest (EcoRI+BamHI) Continued next page

Explanation / Answer

EcoRI digestion: 0.9 kb + 8.7 kb + 10.4 kb

BamHI digestion: 3.2 kb + 16.8 kb

EcoRI + BamHI double digestion: 0.9 kb + 8.7 kb + 1.7 kb + 3.2 kb + 5.5 kb

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