Bio-123 Molecular Biology Part 2 Post Lab 8. EXTRA CREDIT As part of an undergra

Question

Bio-123 Molecular Biology Part 2 Post Lab 8. EXTRA CREDIT As part of an undergraduate research project, a student was attempting to construct a pal, Ncol. and Xbal. After below is obtained, restriction map for the linear DNA fragment Mic2 using the restriction enzymes H carrying out both single and double enzyme digests and electrophoresis the picture showing the number of DNA fragments produced in each digest, along with the sizes of each fragment. From this information, construct a restriction map of Mic2 for these enzymes. Uncut Hpa! Ncol Xbat++ 6318 3242 2223 261 2374 1683 1683 1 295 1295 1079 SL 702 702

Explanation / Answer

From the 5'-end:
Hpa I cuts at 4635 bp
Nco I cuts at 1295 bp
Xba I cuts at 2374 bp and 5616 bp

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