What is one advantage of using a swinging bucket rotors (also known as horizonti

Question

What is one advantage of using a swinging bucket rotors (also known as horizontial rotors when separating biological particles? Swinging bucket rotors (also known as horizontal rotors) have the advantage that there is usually a clean meniscus of minimum area. Since the buckets are easy to interchange, this type of rotor is extremely versatile. o Swinging bucket rotors are smaller than fixed angle or other types of rotors o Swinging bucket rotors have a larger sample volume capacity than foxed angle rotors. o Swinging bucket rotors are less expensive than fixed angle or other types of rotors. The equation we use in the lab to calculate dilutions is: C1 xV1 C2 x V2 Where c is concentration and v is volume. To make a solution with a concentration of C2 and a volume of V2 from a stock with a concentration C1 you just need to put these three numbers into the above equation and solve for V1, the volume of stock you will need to add. Remember to keep track of the units! if you wish to make 50 mls of a 1:10 dilution ofa sample in a buffer then you would add: o 5 mis of the sample to 45 mls of the buffer o 2.5 mis of the sample to 45 mls of the buffer o 10 mls of the sample to 50 mls of the buffer o 10 mis of the sample to 50 ms of the buffer A previous sample was already diluted 1:4. If you then added 1 ml of the diluted sample to 4mi of distilled water, your final sample dilution would be: o t:20 o 1:30 1:40 e 1:s If you have a Mstock of nact and you wish to make 7mof 10 m NaCt

Explanation / Answer

Answer

1. Swinging bucket rotor have a large sample volume capacity than fixed angle rotor.

2. 5 ml of the sample to 45ml of the buffer
Dilution factor = working volume / Total volume
                = 5 / 5 + 45
                = 5 / 50 = 1 : 10

3. 1 : 20

1/ 4 X 1/5 = 1/20

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