The electrophoretic mobility shift assay (EMSA) experiment demonstrated the very

Question

The electrophoretic mobility shift assay (EMSA) experiment demonstrated the very powerful technique for determining protein-DNA interactions. For this purpose the EMSA can be used qualitatively to o identify unknown DNA molecules. o detect DNA RNA interactions o identify specific transcriptional or co-transcriptional factors identify isoenzymes. The purpose of subjecting your BSA protein standard plot data to the least squares statistical method was to o determine intuitively the best fit straight line. e statistically determine the best straight tine through a series of experimental points e determine the overall shape of the 85A standard curve o determie the protein concentration in the unknown samples The advantage of using differential centrifugation to separate components of cell is based cn large differences in slze and shape of these cellular components smalil but measurable differences to size, hee, and density of these eliar components the type of rotor used the consant Finish Test

Explanation / Answer

Q1) C

Q2)D

Q3)B Differential centrifugation is a common procedure in microbiology and cytology used to separate certain organelles from whole cells for further analysis of specific parts of cells. It is based on density, size and shape of organelles.

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