ntioh1i pts) Suppose yau have done E.coli transformation using pGLO plasmid with
ID: 269541 • Letter: N
Question
ntioh1i pts) Suppose yau have done E.coli transformation using pGLO plasmid with the following settings. pGLO plasmid has the following structure, which will be useful for transformant selection process or Match the plasmid components with the following descriptions (2pts each) arac 58. The component to make the plasmid multiply independently p OFP Amp from the genomic DNA. 59. The component transforms . col from antbiotic sensitive to antibiotic resistant 60. The gene expressing activator protein 61. The gene expressing fluorescent protein. 62. Prediction of transformation result (5 pts totall. E co -pGLO E. cow +pGLO plasmidExplanation / Answer
58. In the every plasmid origin of replication site is present that initiate the replication of plasmid. This is representing by “Ori” in plasmid diagram.
59. In Ampr notion represents the symbol for resistance against the ampicillin resistance. This encode for the protein beta-lactamase that cleave the beta lactame ring of the ampicillin. Some time it also represent by ?-lactamase.
60. Activator protein is represented by araC that activate in presence of arabinose sugar and synthesize enzymes that catalyze the arabinose as a substrate.
61. GFP encode for the green fluorescent protein.
For question 62, type of plates is not mentioned. Such as that plate contain ampicillin or not
If they contain ampicilline then only plasmid transformed cells will grow on ampicillin containing media.
If no selection pressure is present then all the plates show colonies.
Please specify the plates then I will answer it.
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