You took 1.0 ml of your cell culture, spun it down a centrifuge, and brought the

Question

You took 1.0 ml of your cell culture, spun it down a centrifuge, and brought the cell pellet up to 100 ul with PBS. You then took 0.1 ml of the new cell suspension and added to it 0.1 ml trypan blue. You counted a total of 235 cells in five big square on a hemacytometer. What is the cell density of your original culture? You took 1.0 ml of your cell culture, spun it down a centrifuge, and brought the cell pellet up to 100 ul with PBS. You then took 0.1 ml of the new cell suspension and added to it 0.1 ml trypan blue. You counted a total of 235 cells in five big square on a hemacytometer. What is the cell density of your original culture?

Explanation / Answer

Answer:- The cell density of the original culture should be 4.7 x 105 cells. 235 cells in five quadrants gets the average of 47 in one quadrant. .i.e 4.7x105 cells in 1 mL of original culture.

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