Protein: Alpha Amylase Enzyme/Protein type: Carbohydrase Metabolic pathway or fu

Question

Protein: Alpha Amylase

Enzyme/Protein type: Carbohydrase

Metabolic pathway or function: Digestion

Source: Meal Worm (Tenebrico molitor)

PBD number: 1CLV

Primary Sequence :

>1CLV:A|PDBID|CHAIN|SEQUENCE

EKDANFASGRNSIVHLFEWKWNDIADECERFLQPQGFGGVQISPPNEYLVADGRPWWERYQPVSYIINTRSGDESAFTDM

TRRCNDAGVRIYVDAVINHMTGMNGVGTSGSSADHDGMNYPAVPYGSGDFHSPCEVNNYQDADNVRNCELVGLRDLNQGS

DYVRGVLIDYMNHMIDLGVAGFRVDAAKHMSPGDLSVIFSGLKNLNTDYGFADGARPFIYQEVIDLGGEAISKNEYTGFG

CVLEFQFGVSLGNAFQGGNQLKNLANWGPEWGLLEGLDAVVFVDNHDNQRTGGSQILTYKNPKPYKMAIAFMLAHPYGTT

RIMSSFDFTDNDQGPPQDGSGNLISPGINDDNTCSNGYVCEHRWRQVYGMVGFRNAVEGTQVENWWSNDDNQIAFSRGSQ

GFVAFTNGGDLNQNLNTGLPAGTYCDVISGELSGGSCTGKSVTVGDNGSADISLGSAEDDGVLAIHVNAKL

>1CLV:I|PDBID|CHAIN|SEQUENCE

CIPKWNRCGPKMDGVPCCEPYTCTSDYYGNCS

Question :

- Describe the primary Sequence in detail (Eg- the number of amino acid residues)

- Describe and label Carboxy and amino termini

- Extra features besides the ends, Label two other regions of the protein and their function

Explanation / Answer

Answer

1)471 residues,Mass (Da):51,240 Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.rotein has several cofactor binding sites: ca2+ and chloride

2)

Carboxy terminal

EKDANFASGRNSIVHLFEWKWNDIADECERFLQPQGFGGVQISPPNEYLVADGRPWWERYQPVSYIINTRSGDESAFTDM

TRRCNDAGVRIYVDAVINHMTGMNGVGTSGSSADHDGMNYPAVPYGSGDFHSPCEVNNYQDADNVRNCELVGLRDLNQGS

DYVRGVLIDYMNHMIDLGVAGFRVDAAKHMSPGDLSVIFSGLKNLNTDYGFADGARPFIYQEVIDLGGEAISKNEYTGFG

CVLEFQFGVSLGNAFQGGNQLKNLANWGPEWGLLEGLDAVVFVDNHDNQRTGGSQILTYKNPKPYKMAIAFMLAHPYGTT

RIMSSFDFTDNDQGPPQDGSGNLISPGINDDNTCSNGYVCEHRWRQVYGMVGFRNAVEGTQVENWWSNDDNQIAFSRGSQ

GFVAFTNGGDLNQNLNTGLPAGTYCDVISGELSGGSCTGKSVTVGDNGSADISLGSAEDDGVLAIHVNAKL amino terminal

3)DOMAIN 291-341  BPTI/Kunitz inhibitor : active domains of proteins that inhibit the function of protein degrading enzymes

MOTIF 759-762 NPXY motif; contains endocytosis signal

Metal binding position of amino acid for calcium 98,146,155 and 189

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