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In the background section the authors wrote “Organs and tissues of zebrafish are

ID: 271109 • Letter: I

Question

In the background section the authors wrote “Organs and tissues of zebrafish are similar to those of humans in structure and function, and regulation of energy homeostasis at the neural and endocrine level is conserved.” What does the word conserved mean in this sentence? (1 point)

In the “Zebrafish husbandry” section of the methods section the authors wrote “Three independent feeding experiments were performed over a period of 18 months.” What were these three independent feeding experiments? (1 point)

In the “Zebrafish husbandry” section of the methods section the authors wrote “Blood samples were centrifuged and plasma was collected from supernatant.” What would have been missing in the supernatant? (1 point)

What kind of ratio is there between the points on the x axis and the points on the y axis to generate a line? (1 point)

How do you know that your answer to question 4 is correct? (1 point)

In the results section the authors wrote “While fish in the NFD-OF group almost doubled their weight, the weight gain of HFD-OF fish was not as prominent (Fig. 2c).” In which week was it really not as prominent? (1 point)

How do you know that your answer to question 6 correct? (1 point)

In the results section the authors wrote “Body fat percentage was increased in both NFD-OF and HFD-OF fish but did not significantly differ between the OF groups (Fig. 3).” What does OF stand for? (1 point)

In the results section the authors wrote “In subcutaneous AT adipocyte number was significantly increased in NFD-OF fish, while there was only a marginal increase in adipocyte number in HFD-OF fish (Fig. 3h).” Why would subcutaneous AT adipocyte number be significantly increased in NFD-OF fish but only marginally increased in HFD-OF fish? (1 point)

Why were male zebrafish used but not female zebrafish? (1 point)

Reference: https://bmcphysiol.biomedcentral.com/articles/10.1186/s12899-017-0031-x

Explanation / Answer

Ans:- conserved in case of nucleotide sequences are those sequences which are similar in different organism even though they belonging to different species groups.

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