Could you use yeast cells instead of bacteria as a host for replication of your
ID: 271195 • Letter: C
Question
Could you use yeast cells instead of bacteria as a host for replication of your asmid DNA? (YN) Why? What do you expect to be the difference between the undiluted plate and the 1:10 dilution plate of bacteria after overnight incubation? How would you prepare a 1:100 dilution of bacteria? 31 w would you prepare a 1:1000 dilution? ou purified 5 g of your plasNd DNA ¡/total, what would the concentration of DNA in your final plasmid prep colleaon tule (HINT: Refer to step #18 in "Extracting A from Bacteria" protocol to determiné the concentration) e space below if additional space to write notes is needed.]Explanation / Answer
Answer:
Could you use yeast cells instead of bacteria as a host for replication of your plasmid DNA? Why?
What do you expect to be the difference between the undiluted plate and the 1:10 dilution plate of bacteria after overnight incubation?
The growth in the undiluted plate will be more as compared to the 1:10 dilution plate. This is because the initial cell number will be higher in undiluted plate and when cells divide and grow exponentially the population of undiluted plate will be higher after overnight incubation.
How would you prepare a 1:100 dilution of bacteria?
How would you prepare a 1:1000 dilution?
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