Can you answer these Qs about Cell suspension and cell washing ? Lab Exercise 1:

Question

Can you answer these Qs about Cell suspension and cell washing ?

Lab Exercise 1: Cell Washing and Cell PAGE 6 of 6 Suspension Questions 1. How long are red cell suspensions good for? Why? 2. What is the optimum percent cell concentration for blood bank testing? Why? 3. List three "do's" and "don'ts" of cell washing. 4. What is the principle of the sulfosalicylic acid test performed on the supernatant of the cells being washed? 5. Describe the proper technique for adequately washing of red blood cells. 6. Name four constituents that are removed during red cell washing. 7. Why would cord bloods require more washing?

Explanation / Answer

I'm only answering first four as per the rules of Chegg.

1. Red cell suspension can be stored at 2–6 °C for a period of 5-6 weeks depending on the red cell preservation solution used. During ex-vivo storage, red cells undergo changes affecting function and viability, often collectively referred to as ‘storage lesions’, that’s why RBC suspension cannot be stored for very long.

2. The optimum percent cell concentration for blood bank testing is between 2 – 5%. The ratio of serum to cells markedly affects the sensitivity of agglutination tests. Preparation of a 2-5% cell suspension provides cells in an optimum concentration to detect weak antibodies.

3. Do’s and Don’ts of cell washing-

  Do’s - a) Cell should be centrifuged long enough to pull most of cells in the bottom of the tube, b) Decant the saline completely, c) Shake the tube to resuspend cell pallet at the bottom of the tube before washing the cells again

    Don’ts – a) Do not take more than 3-4 drops of blood in the tube for centrifugation, b) Do not slowly add saline to the blood, use squirt bottle to forcibly add it to blood, c) Do not fill more than 3/4th of the tube with saline and blood.

4. Sulfosalicylic acid test is performed to detect and quantify the presence of protein in the supernatant. If protein is present, the solution will become turbid. The degree of turbidity is used to quantify the amount of protein present in the supernatant.

Thanks!

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