3A. What conclusion do you draw from this figure? A. SON inhibits ESC differenti

Question

3A. What conclusion do you draw from this figure?

A. SON inhibits ESC differentiation and inhibits Oct4 splicing

B. SON inhibits ESC differentiation and activates Oct4 splicing

C. SON ctivates ESC differentiation and inhibits Oct4 splicing

D. SON activates ESC differentiation and activates Oct4 splicing

3B. What are two additional conclusions you draw from the data?

mRNA processing and ESCs Wild SON type knockdown 12 e 15r Blue = wt Oct4 is arn ESC required txn factor Bright field 3 0 Alkaline phosphatase Alkaline phosphatase is an ESC marker

Explanation / Answer

A. SON inhibits ESC differentiation and inhibits Oct4 splicing.

High level Alkaline Phosphatase expression is the characteristic feature of undifferentiated state of ESCs. Since knocking down SON has resulted in loss of Alkaline Phosphatase, it indicates that SON inhibits ESC differentiation. Also, SON knockdown (SON KD) has increased Oct4-intron 4 enrichment which indicates inhibition of splicing as intron is not removed.

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