ingerprinting, why are radioactive probes uned after the Souatherm blo? Only spe
ID: 272336 • Letter: I
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ingerprinting, why are radioactive probes uned after the Souatherm blo? Only specific nucleotide sequences can be highlighted using probes The DNA is too small to be visualized without the probes 6DNA cannot be visualized without the probes. The restriction enzymes will not work without the probes. All of these are trae. Which option BEST describes sticky ends. G. Sticky ends are DNA fragments that 62) by restriction enzymes carry a higher charge than normal afher they have been cleaved are DNA fragments eleaved by a restriction enryme so thust one strand is longer than other Sticky ends are DNA fragments length Sticky ends are DNA fragments by a restriction enzyme eleaved by a restriction enryme so that both strands are the same that atract a carbohydrate molecule to one end after being cleaved 63) In nature, the purpose of restriction enzymes is to protect the bacterium protect the bacterium from the DNA of other organisms the bacteriurin protect the bacterium from replicating its DNA at the wrong time. protect the bacterium from virus attack by cutting up toreigh virus atack by not allowing the virus to attach to the cell wall organisms the bacterium infects DNA lab in class? 64) Which of the following were used in the restriction enxyme a. EcoRI b. Lambda phage DNA c. plasmid DNA d. HindIlI e. All of these were used. 65) Based on the above DNA fingerprint, the soldier was the son of which set of the parents? a) A & B b) C&D; c) E&F; d)G&H;Explanation / Answer
61)Answer is option B
Only specific nucleotide sequence is highlighted using probes.
As the radioactive probe binds to the DNA fragment that has the matching sequence and no other spacer or junk DNA.
Thus it helps in which specific sequence can be seen at the end of process.
62)Answer is option B
Sticky ends are the DNA fragments that are cleaved by restriction enzyme,so that there is one strand longer than the other.
When restriction enzyme cleaves it leaves sticky ends and blunt ends.
Sticky ends has protruding single strand with unpaired nucleotides called overhangs,which combine with their complementary strands.
63)Answer is option D
In nature, restriction enzymes in bacterium acts as a part of defence mechanism and protect from virus by cutting foreign DNA.
These enzymes in prokaryotes cut the foreign DNA by RESTRICTION.
65)Answer is option B
C and D as the more likely to be parents as the VNTR are more related to soldier( variable number tandem repeats).
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