7. A eukaryotic structural gene has two introns and three exons: S\'-exon1-intro

Question

7. A eukaryotic structural gene has two introns and three exons: S'-exon1-intron -exon2-intron2-exon3-3' The GU at the 5' end of intron2 has been mutated so it is no longer recognized. What would the mature mRNA look like in the mutant? (A) S-exon1-exon2-exon3-3 (B) S'-exon1-intron 1-exon2-exon3-3' (C) 5-cxon1-exon2-intron2-exon3-3 (D) 5'-exonl-intron1-exon2-3 (E) 5-exon2-intron2-exon3-3 8. In prokaryotes, a promoter has consensus sequences typically located at and (A) -25, -75 (B) -25,-35 (C)-10,-25 (D)-10, -35 CE (E)-10,-75 9. The protein responsible for binding the consensus sequences in question 8 is (A) beta factor (B) sigma factor (C) TBP (D) alpha factor (E) none of the above 10. Base-pair substitutions involving the third base of a codon are unlikely to result in an error in the polypeptide. Thi is because (A) substitutions are corrected before transcription begins (B) substitutions are restricted to introns (C) the base-pairing rules are less strict for the third base of codons and anticodons (D) a signal-recognition particle corrects coding errors (E) transcribed errors attract snRNPs, which then stimulate splicing and correction 11. Lagging strand synthesis involves (A) Okazaki fragments (B) Shine-Dalgarno fragments (C) Klenow fragments (D) restriction fragments

Explanation / Answer

Answer:

7) What would the mature mRNA look like in the mutant?

8) In prokaryotes, a promoter has consensus sequence typically located at -10 and -35  

9) The protein responsible for binding the consensus sequences is

10) Base-pair substitutions involving the third base of a codon are unlikely to result in an error in the polypeptide. This is because

11) Lagging strand synthesis involves

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