Short answer questions; Write the function of SDS and Mercaptoethanol in SDS-PAG

Question

Short answer questions; Write the function of SDS and Mercaptoethanol in SDS-PAGE (3 marks) #spS 'y gives tne protein negative chor 67e binolina to i ?ercaotoethanol s is a buffer used as Solulate tw 2. Write the commands to visualize disulfide bonds in a protein by using RASMOL(3 marks) 3. Using RasMol; to visualize secondary structure in colour, what are the commands(3 marks) Write the two membranes we can use to transfer protein and its differences (3 marks) #Asiho cellulae -git needs methanol 4. PVDE ? the gel does not need wethou4 Bat the membrave neeeds it

Explanation / Answer

Ans 2:  RasMol>ssbonds

simply typing “ssbonds” will only shows dotted lines. To give these bonds dimension, we must add a value (thickness) to the ssbonds. Example: RasMol>ssbonds 225

and for color : RasMol>color ssbond orange

Ans3:

RasMol>select helices

RasMol>color red

these commands will color only helicels, for beta sheets write

RasMol>select sheets

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