e tuney ared to ake recombinant DNA) True or False [Fill in (a) for True and (b)
ID: 272975 • Letter: E
Question
e tuney ared to ake recombinant DNA) True or False [Fill in (a) for True and (b) for False] 22. The primary structure of a protein is directed by the genetic sequence encoding that proteir 23. Restriction endonucleases are enzymes that cut DNA at specific double-stranded sequences 24. In PCR, heat is used to denature (separate) DNA strands 25. Taq polymerase is used in PCR because it is stable at high temperatures. 26. A bacteriophage, a kind of virus that infects bacteria, can be used as a cloning vector. 7. DNA ligase is an enzyme that generates "sticky ends." 8. Enzymes work by increasing activation energy (the energy required to start a chemical reaction). 9. Eukaryotic genes can be expressed in bacteria using an appropriate promoter. . Human insulin is an example of a product produced by molecular cloning. Selective breeding leading to the domestication of animals is an example of ancient bioengineering. Biotechnology began with the discovery of the double helical structure of DNA.Explanation / Answer
23.True
The primary structure of a protein is simply the sequence of amino acids. This structure is dictated by the information encoded with DNA and the mRNA copy.
24. True
Restriction enzymes recognize a specific sequence of nucleotides and produce a double-stranded cut in the DNA.
25. True
High temperature is used repeatedly in PCR to denature the template DNA, or separate its strands.
26. True
Taq polymerase is stable in high temperature. It is isolated from heat-tolerant bacterium, Thermus aquaticus.
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