18. What are some applications for using a growth curve? 19. List three ways to

Question

18. What are some applications for using a growth curve? 19. List three ways to estimate the size of a population of bacteria ?.3. 20. What does the increase in turbidity represent when using a spectrophotometer? 21. What is a drawback of the direct cell count? 22. How does a Coulter counter count the number of cells in a population? 23. Why is a viable plate count more accurate than a Coulter counterestimate? 24. When is counting bacteria by filtration used? of when counting bacteria by filtration is used?

Explanation / Answer

18) Knowledge of bacterial growth kinetics is useful for several purpose. From the research point of view it is often critical to know the number of bacterial cell in a sample, so the experiment can be repeated several times without any major deviation in values. From the commercial point of view we can know the how much inoculum needed to harvest desired metabolites. Growth kinetics can also inform researcher whether a particular bacteria can successfully able to metabolize toxic substances & thus minimize the pollution.

19) Bacterial population can be estimated by both direct & indirect methods.

Direct methods- Viable plate counts, Membrane filtration, Microscopic counts, Most Probable Number

Indirect methods- Metabolic activity, Dry weight, Turbidity

20) Bacterial growth estimation by turbidity is an indirect method. Turbidity is inversely proportional to the bacterial growth. More clear solution indicate lower bacterial population & vice versa. So, cloudy (increase in turbidity) broth indicates the greater bacterial population.

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