Your group is testing the effect of increasing PCR cycles. After extracting your
ID: 273279 • Letter: Y
Question
Your group is testing the effect of increasing PCR cycles. After extracting your DNA and designing a primer set to amplify a stable, well studied gene to test your hypothesis, you added all the reagents according to a peer-reviewed protocol that has worked in the past. The template was amplified for either 40, 60, or 72 cycles. You ran two sets of experiment: A) the template was not purified, and B) the template was purified to remove unwanted products-- primers, protein, salt, and co-factors. Aliquots (8 ul) of the products were analyzed on a 3% agarose gel below. Summarize results of your experiment.
unpurified purified MWM 40 60 72 40 60 72 MWMExplanation / Answer
It's evident from the gel that DNA replicated 40 times is intact wether purified or not. However, the increasing number of cycles degrade the DNa as a streak is observed instead of proper intact brand.
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