Help with question 6. 5. Draw a labeled diagram show ing the interactions betwee

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Help with question 6.

5. Draw a labeled diagram show ing the interactions between, and relative locations detee of, the target protein, the primary antibody, the secondary antibody, and the tarse horseradish peroxidase once your blot is ready for the final detection step. In addition, for each antibody, label its variable region (V) and its constant region H2P penxidure CAH?) primary an hbud 6. Briefly describe, in words, the reaction that allows you to detect (and visualize) the location of the secondary antibody in your blot and, as a result, the location of the target protein.

Explanation / Answer

Ans 6:- since the secondary antibodies are attached to the floresent agent, Secondary antibody is especially efficient in immunolabeling. Secondary antibodies bind to primary antibodies, which are directly bound to the target antigen(s). In immunolabeling, the primary antibody's Fab domain binds to an antigen and exposes its Fc domain to secondary antibody. Then, the secondary antibody's Fab domain binds to the primary antibody's Fc domain. Since the Fc domain is constant within the same animal class, only one type of secondary antibody is required to bind to many types of primary antibodies. This reduces the cost by labeling only one type of secondary antibody, rather than labeling various types of primary antibodies. Secondary antibodies help increase sensitivity and signal amplification due to multiple secondary antibodies binding to a primary antibody

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