answer question 5/22/2018 6 Seeding wells with cells After trypsinizing your cel
ID: 273364 • Letter: A
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5/22/2018 6 Seeding wells with cells After trypsinizing your cells you have a concentration of 350,000 cells/ml in a total volume of 10 ml. How many total cells do you have? You want to make various combinations of culture media, extracellular matrix coating, and cell densities to test the hypothesis that different cell densities on different ECM affects stem cell differentiation. Mudio Coah Expansion FN 25,000 cells 3 replicates of each Osteogenic Collagen I 50,000 cells Nothing 100,000 cells Do you have enough cells? How will you set up the experiments using 12 well plates? Notes: Cell dilutions can be made in media and you will only add 1 ml cell suspension to each well for seeding. ading inhibitors to cellsExplanation / Answer
Answer:
6)
Total number of cells required for test the hypothesis
96 Well Plate Experiment:
1) First make following concentrations of cell by diluting the stock ( 350,000 cell per ml), total volume 10ml
100000 cells/ml:
50,000 cells/ml:
25,000 cells/ml
Now we have required volume and three different concentration of cells and enough sample to test these concentrations in triplicate (total 3.5ml minimum per concentration and 1ml required for each concentration).
Plate Layout of a 96-well plate setup for the experiment:
1 2 3 4 5 6 7 8 9 10 11 12 A 100,000cells/ml 50,000 cells/ml (Collagen 1 Coating) 25,000 cells/ml (FN Coating) B 100,000cells/ml 50,000 cells/ml (Collagen 1 Coating) 25,000 cells/ml (FN Coating) C 100,000cells/ml 50,000 cells/ml (Collagen 1 Coating) 25,000 cells/ml (FN Coating) D E Media Blank Media Blank Media Blank F G HRelated Questions
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