otherapy e of the above wich is NOT true about Gel electrophoresis: )it is used
ID: 273368 • Letter: O
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otherapy e of the above wich is NOT true about Gel electrophoresis: )it is used to separate proteins as part of a Western Blot Analysis b) It is used to separate DNA as part of a Southern Blot Analysis c) It is used to separate RNA as part of a Northern Blot Analysis d) It is used to separate restriction fragments in DNA Fingerprinting e) None of the above (All of the statements are true). 36. Which of the following statements is NOT true regarding CRISPR-CAS9 a) It is derived from a naturally occurring enzyme system found in bacteria b) It provides bacteria with a kind of "molecular memory" of the virus that infected it. c) It can be "programmed" to carry out specific changes to DNA in living cells d) It is regularly used to produce "designer bables." e) None of the above (All of the statements are true). 37. Given the short DNA anticodon sequence: TAC GGC AGC TTG a. The RNA transcript would be: AUG CCG UCG AAC b. The RNA transcript would be: UAC GGC AGC UUG c. The RNA transcript would be: ATG CCG TCG AAC d. None of the above 38. You are a lab tech working in forensics for the NYPD. You are given a hair sample recovered from a anograms of crime scene. You are asked to produce a DNA profile (fingerprint). You recover a few n DNA from the hair sample. What is the next step to producing a DNA fingerprint? a. Restriction digest b. Gel electrophoresis C. Polymerase Chain Reaction (PCR) to amplify the DNA d Southern Blot" (transference of DNA onto nitrocellulose membrane) e. None of the above (all are used for DNA fingerprinting) of DNA fingerprinting? a. Identification of human remains b. Paternity determination c. Crime Scene Investigation d. Using genetic markers to diagnose certain diseases e. None of the above (All are applications of DNA fingerprinting techniques) 40. Which is NOT a true statement regarding GMOs? a. GMO crops can be engineered to produce pesticides b. GMO crops can be engineered to be resistant to drought c. GMO crops can be engineered to contain nutrients not normally found in that food plant d. The long-term effects of consuming GMO's remains unknown e. None of the above (All are true statements)Explanation / Answer
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35. Which of the following is not true about Gel electrophoresis
Answer: Option E. (None of the above (All of the statements are true)
Explanation. Gel electrophoresis is the technique used to separate the charged biomolecules according to their size in the presence of electric field. It is used in for proteins, DNA, RNA in Western, Southern, Northern blotting as well as used for the separation of DNA fragments in DNA fingerprinting.
36. Which of the following statement is not true regarding CRISPER –CAS9
Answer: Option E (All the statements are true)
Explanation: The CRISPR/Cas stands for (Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR associated nucleases) system which was originally discovered to be an acquired immune response mechanism used by archaea and bacteria. This system is used as molecular tool to bring required changes in the DNA sequence of gene. It is one of the natural occurring enzyme system found in bacteria.
37. Given the short DNA anticodon sequence TAC GGC AGC TTG
Answer: Option A (AUG CCG UCG AAC)
38. You are a lab teach working in forensics for the NYPD. You are given a hair sample recovered from a crime scene. You are asked to produce a DNA profile (fingerprint). You recover a few Nanograms of DNA from the hair sample. What is the next step to producing a DNA fingerprint
Answer: Option C (Polymerase chain reaction to amplify the DNA)
Explanation: As DNA quantity is very less and not enough for whole procedure so the first step after DNA isolation will be the PCR amplification of the isolated DNA so that the sufficient amount of DNA can be produced to run the subsequent reactions. In the option all steps of the DNA fingerprinting protocol is given but the PCR amplification is the first step after DNA isolation.
39. Which is not an application of DNA fingerprinting?
Answer: Option E (All are the application of DNA fingerprinting technique)
40. Which is not the true statement regarding GMOs
Answer: Option E None of the above (all statement are true)
Explanation: GMOs are the genetically modified organisms they are used to produce their own pesticides, these pesticides are chemicals which have a negative effect on human beings. They can be engineered to produce drought resistant and high nutrition containing foods.
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