A digital ELISA experiment was used to quantify the concentration of antigen in

Question

A digital ELISA experiment was used to quantify the concentration of antigen in a 100 uL sample prepared with 10,000 magnetic beads decorated with capture antibodies, excess detection antibodies with biotin tags, and excess streptavidin tagged enzymes (analogous to Figure 3 in the reading). After rinsing the magnetic beads, 100 were selected and each sealed into an individual well with enzyme substrates that yield fluorescent products. After incubation, a fluorescence microscope was used to image the 100-well plate. Wells are either fluorescent (0) or dark (): 2 3 4 9 10 1 -WN- 0 ••• ••• - --

Explanation / Answer

a)  

b)

c)

e)

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