Question 10 [15 marks / 15 minutes] Indicate the level of expression of the lac
ID: 275457 • Letter: Q
Question
Question 10 [15 marks / 15 minutes] Indicate the level of expression of the lac operon in the various mutants shown in the table below (only relevant parts of the genotype are shown). You must answer in your answer book No lactose Lactose presentLactose present Escherichia coli genotype (no glucose)Glucose presentNo glucose high 1. wildtype 2. lacP (affects lac operon promoter) 3. lacI 4. lacIS 5. laclS lacO 6. lacI+/ lacIs (partial diploid) 7. CAP gene 8. CAP gene- ladc 9. CAP genelaclS 10. CAP gene CAP gene Mone low (partial diploid)Explanation / Answer
No lactose
No glucose
Lactose present
Glucose present
Lactose present
No Glucose
CAP gene- lac Is
CAP gene-/CAP gene+
No
No
No
Low
No
High
GenotypeNo lactose
No glucose
Lactose present
Glucose present
Lactose present
No Glucose
Wild Type No Low High lac P- No No No lac I- No High High lac Is No N9 No lac Is Oc No High High lac I+/ lac Is No No No CAP gene- No High High CAP gene- lac Oc No High HighCAP gene- lac Is
CAP gene-/CAP gene+
No
No
No
Low
No
High
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